Cotmore S F, Tattersall P
Science. 1984 Dec 7;226(4679):1161-5. doi: 10.1126/science.6095448.
The genome of the small human virus serologically associated with erythrocyte aplasia and erythema infectiosum (fifth disease) is shown to be a linear, nonpermuted, single-stranded DNA molecule with self-priming hairpin termini, properties which are characteristic of the genomes of the family Parvoviridae. This human parvovirus chromosome was molecularly cloned into bacterial plasmid vectors and the cloned DNA was used to explore its relatedness to other mammalian parvovirus serotypes by DNA:DNA hybridization. It is not related to the human adeno-associated viruses but does show a distant evolutionary relationship to genomes of the helper-independent parvoviruses of rodents. This strongly suggests that it is an autonomous parvovirus, and as such is the first example of a member of this group of common animal pathogens to cause disease in man.
与红细胞再生障碍和传染性红斑（第五病）血清学相关的小型人类病毒的基因组被证明是一个线性、未重排、具有自我引发发夹末端的单链DNA分子，这些特性是细小病毒科基因组的特征。这种人类细小病毒染色体被分子克隆到细菌质粒载体中，克隆的DNA被用于通过DNA:DNA杂交来探索它与其他哺乳动物细小病毒血清型的相关性。它与人类腺相关病毒无关，但确实与啮齿动物的非依赖辅助病毒的细小病毒基因组显示出远缘进化关系。这有力地表明它是一种自主细小病毒，因此是这类常见动物病原体中导致人类疾病的首个成员实例。
