Merchlinsky M J, Tattersall P J, Leary J J, Cotmore S F, Gardiner E M, Ward D C
J Virol. 1983 Jul;47(1):227-32. doi: 10.1128/JVI.47.1.227-232.1983.
The linear single-stranded DNA genome of minute virus of mice, an autonomous parvovirus, was cloned in duplex form into the bacterial plasmid pBR322. The recombinant clones of minute virus of mice were infectious when transfected into monolayers of human 324K cells and produced virus plaques with an efficiency of about 6% that obtained with duplex replicative-form DNA purified from cells infected with minute virus of mice. Southern blot analysis of transfected cells indicated that the cloned minute virus of mice genome requires both termini to be intact for excision and replication as a linear duplex molecule.
小鼠微小病毒（一种自主细小病毒）的线性单链DNA基因组以双链形式克隆到细菌质粒pBR322中。当将小鼠微小病毒的重组克隆转染到人324K细胞单层中时具有感染性，并产生病毒斑，其效率约为从小鼠微小病毒感染的细胞中纯化的双链复制型DNA所获得效率的6%。对转染细胞的Southern印迹分析表明，克隆的小鼠微小病毒基因组需要两个末端均完整，才能作为线性双链分子进行切除和复制。
