Deiss V, Tratschin J D, Weitz M, Siegl G
Institute for Hygiene and Medical Microbiology, University of Berne, Switzerland.
Virology. 1990 Mar;175(1):247-54. doi: 10.1016/0042-6822(90)90205-6.
We describe the molecular cloning of the entire 5.6-kb single-stranded DNA genome of the human parvovirus B19 in bacterial plasmids. Stable amplification of the recombinant plasmid DNA was achieved in Escherichia coli JC8111 but not in HB101 cells. Sequence analysis of the cloned DNA shows that the terminal 383 nucleotides at each end of the genome are identical inverted repeats. The distal 365 nucleotides of the repeat represent an imperfect palindrome which presumably folds over to form a hairpin structure. The sequence of the hairpin occurs in two distinct configurations which are related in that one is the inverted complement of the other. Such alternative configurations of the terminal hairpins have been found for all parvoviruses analyzed so far and are referred to as flip and flop.
我们描述了人类细小病毒B19完整的5.6kb单链DNA基因组在细菌质粒中的分子克隆。重组质粒DNA在大肠杆菌JC8111中实现了稳定扩增，但在HB101细胞中未实现。对克隆DNA的序列分析表明，基因组两端的末端383个核苷酸是相同的反向重复序列。重复序列远端的365个核苷酸代表一个不完全回文序列，推测其折叠形成发夹结构。发夹序列以两种不同的构型出现，二者相关，其中一种是另一种的反向互补序列。到目前为止，在所有分析过的细小病毒中都发现了末端发夹的这种交替构型，被称为翻转和反转。
