DeVries P J, Davidson R L, Clough D W
Somat Cell Mol Genet. 1984 Nov;10(6):625-32. doi: 10.1007/BF01535228.
A line of mouse cells containing a methylated inactive herpes simplex virus thymidine kinase (TK) gene was irradiated with ultraviolet (UV) light in an attempt to induce expression of the inactive TK gene. UV irradiation was shown to be capable of inducing expression of the viral TK gene in a dose-dependent manner. Analysis of the methylation pattern of the viral TK gene indicated that the active TK gene in three UV-induced TK+ cell line was methylated to a lesser extent than was the inactive viral TK gene in the parental cells. Analysis of the copy number of the viral TK gene in parental and UV-induced cell lines also revealed that the viral TK gene was amplified 3- to 20-fold in three of four UV-induced TK+ cell lines tested.
为了诱导甲基化的无活性单纯疱疹病毒胸苷激酶（TK）基因表达，对含有该基因的小鼠细胞系进行了紫外线（UV）照射。结果表明，UV照射能够以剂量依赖的方式诱导病毒TK基因的表达。对病毒TK基因甲基化模式的分析表明，在三个UV诱导的TK+细胞系中，活性TK基因的甲基化程度低于亲代细胞中无活性病毒TK基因的甲基化程度。对亲代细胞系和UV诱导细胞系中病毒TK基因拷贝数的分析还显示，在四个测试的UV诱导TK+细胞系中，有三个细胞系的病毒TK基因扩增了3至20倍。
