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[青蛙光感受器细胞质膜的钾离子电导率]

[Potassium conductivity of the plasma membrane of frog photoreceptor cells].

作者信息

Kolesnikov S S, Liubarskiĭ A L

出版信息

Biofizika. 1984 Sep-Oct;29(5):786-9.

PMID:6095924
Abstract

Potassium channels, uniformly distributed over the cell surface (0.5 channel/micron2) have been found in isolated fragments of plasma membranes of frog photoreceptor cells. Their conductance under symmetrical conditions (0.1 M KCl at both the membrane surfaces) is 72 +/- 6 pS for rods and 88 +/- 8 pS for cones. The channels are reversibly blocked by tetraethylammonium (TEA), Cs+, Rb+ at intra- and extracellular membrane surfaces. Half-inactivation of a channel occurs at concentrations of TEA, Cs+ and Rb+ at the intracellular surface 0.12, 2.2 and 3.9 mM, correspondingly.

摘要

钾通道均匀分布于细胞表面(每平方微米0.5个通道),已在蛙感光细胞质膜的分离片段中被发现。在对称条件下(膜两侧均为0.1M氯化钾),视杆细胞通道的电导为72±6皮西门子,视锥细胞通道的电导为88±8皮西门子。这些通道在细胞内膜表面和细胞外膜表面均可被四乙铵(TEA)、铯离子(Cs +)、铷离子(Rb +)可逆性阻断。细胞内表面的TEA、Cs +和Rb +浓度分别为0.12 mM、2.2 mM和3.9 mM时,通道发生半数失活。

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