Sato C, Nishizawa K, Yamaguchi N
Cell Struct Funct. 1984 Sep;9(3):305-9. doi: 10.1247/csf.9.305.
Rat SV-3Y1 cells were stained with double immunofluorescence. Treatment of cells with detergent and salt removed about 80% of the antigens and revealed immunofluorescent flecks of the SV40 large T antigen and p53 bound to the nuclear skeleton. These flecks exactly corresponded to the fluorescent spots produced by antibodies against microtubule associated protein-1. The MAP-1 analogues may function in the initiation of DNA synthesis through the interaction with T-antigen and p53.
用双重免疫荧光法对大鼠SV - 3Y1细胞进行染色。用去污剂和盐处理细胞可去除约80%的抗原，并显示出与核骨架结合的SV40大T抗原和p53的免疫荧光斑点。这些斑点与抗微管相关蛋白-1抗体产生的荧光斑点完全对应。MAP - 1类似物可能通过与T抗原和p53相互作用在DNA合成起始过程中发挥作用。
