Sato C, Nishizawa K, Nakamura H, Ueda R
Exp Cell Res. 1984 Nov;155(1):33-42. doi: 10.1016/0014-4827(84)90765-1.
Monoclonal antibody against microtubule-associated protein-1 produced intranuclear immunofluorescent spots, which disappeared under growth-inhibited conditions caused by serum starvation and saturated cell density in untransformed cells. A change of medium to 10% serum gave rise to the reappearance of nuclear spots before the resumption of DNA synthesis. This reversible change of immunofluorescence was also caused by a temperature shift in rat 3Y1 cells transformed by Simian virus-40-A640 (temperature-sensitive in large T-antigen). The fluorescence decreased during S phase of the cell cycle. In contrast the transformed cells always showed nuclear fluorescence, irrespective of serum concentrations or the cell cycle. Growth-inhibited cells previously treated with detergent and salt revealed nuclear fluorescent spots. This result suggested antigenic modification.
针对微管相关蛋白-1的单克隆抗体产生了核内免疫荧光斑点，在未转化细胞中，血清饥饿和饱和细胞密度导致的生长抑制条件下，这些斑点消失。将培养基换成10%血清会导致在DNA合成恢复之前核斑点重新出现。这种免疫荧光的可逆变化也由猿猴病毒40-A640（大T抗原温度敏感）转化的大鼠3Y1细胞中的温度变化引起。在细胞周期的S期荧光降低。相比之下，转化细胞无论血清浓度或细胞周期如何，总是显示核荧光。先前用去污剂和盐处理过的生长抑制细胞显示出核荧光斑点。这一结果提示了抗原修饰。
