Gontero B, Meunier J C, Buc J, Ricard J
Eur J Biochem. 1984 Dec 17;145(3):485-8. doi: 10.1111/j.1432-1033.1984.tb08582.x.
A slow conformation change of chloroplastic reduced fructose bisphosphatase is detected upon raising the pH from 7 to 8 or upon lowering the pH from 8 to 7. These conformation changes are fully reversible. In the time scale investigated these processes occur in one step. Their time constants and their amplitudes have been determined and analyzed as a function of proton concentration. The results obtained are consistent with the view that upon ionization or protonation of a strategic ionizable group the protein undergoes a 'slow' conformational transition that may be followed by conventional fluorescence techniques. Since illumination brings about a pH rise of chloroplastic stroma from 7 to 8, the above results suggest that light activation of fructose bisphosphatase is at least in part due to a slow conformation change of this enzyme.
当将pH从7提高到8或从8降低到7时，可检测到叶绿体中还原型果糖二磷酸酶的缓慢构象变化。这些构象变化是完全可逆的。在所研究的时间尺度上，这些过程一步发生。已经确定并分析了它们的时间常数和幅度作为质子浓度的函数。所获得的结果与以下观点一致：在一个关键的可电离基团发生电离或质子化时，蛋白质会经历一个“缓慢”的构象转变，这可以通过传统的荧光技术来跟踪。由于光照会使叶绿体基质的pH从7升高到8，上述结果表明果糖二磷酸酶的光激活至少部分是由于该酶的缓慢构象变化。
