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质粒克隆载体pBR322的高拷贝数衍生物。

High-copy-number derivatives of the plasmid cloning vector pBR322.

作者信息

Boros I, Pósfai G, Venetianer P

出版信息

Gene. 1984 Oct;30(1-3):257-60. doi: 10.1016/0378-1119(84)90130-6.

DOI:10.1016/0378-1119(84)90130-6
PMID:6096220
Abstract

A stable copy-number mutant of a pBR322-derived plasmid was isolated. The mutation was found to be a single G----T transversion located near the 3' end of a DNA segment coding for the regulatory RNA I. The resulting copy number for this plasmid is approx. 1000 per cell or 65% of total cellular DNA. Several cloning vectors have been constructed from this copy-number mutant and their practical application is discussed.

摘要

分离出了一种源自pBR322质粒的稳定拷贝数突变体。发现该突变是一个位于编码调控RNA I的DNA片段3'端附近的单碱基G到T的颠换。这种质粒产生的拷贝数约为每个细胞1000个，占细胞总DNA的65%。已从这种拷贝数突变体构建了几种克隆载体，并讨论了它们的实际应用。

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