用放射免疫电泳法对不同细胞系中EB病毒核抗原进行鉴定。
Characterization of Epstein-Barr virus nuclear antigen(s) in different cell lines by radioimmunoelectrophoresis.
作者信息
Sculley T B, Spelsberg T C, Pearson G R
出版信息
Intervirology. 1984;22(4):191-200. doi: 10.1159/000149551.
Utilizing radioimmunoelectrophoresis, Epstein-Barr virus nuclear antigens (EBNA) with molecular weights of 67,000 and 70,000 were detected in NC37 cells, while the Raji cell line contained an antigen with a molecular weight of 70,000. Cell lines containing the B-95 strain of Epstein-Barr virus (EBV) expressed antigens with a molecular weight of 73,000. Three different B-lymphocyte cell lines, established by infection with the B-95 virus, also exhibited 73,000 molecular weight EBNA proteins. Cell lines containing the P3HR-1 strain of EBV demonstrated a single antigen with a molecular weight of 72,000. The results indicate that the molecular weight of EBNA is determined by the strain of infecting virus.
利用放射免疫电泳法，在NC37细胞中检测到分子量为67,000和70,000的爱泼斯坦 - 巴尔病毒核抗原（EBNA），而拉吉细胞系含有一种分子量为70,000的抗原。含有爱泼斯坦 - 巴尔病毒（EBV）B - 95株的细胞系表达分子量为73,000的抗原。通过感染B - 95病毒建立的三种不同的B淋巴细胞系也显示出分子量为73,000的EBNA蛋白。含有EBV P3HR - 1株的细胞系显示出一种分子量为72,000的单一抗原。结果表明，EBNA的分子量由感染病毒的毒株决定。
Zotero 插件