Strnad B C, Schuster T C, Hopkins R F, Neubauer R H, Rabin H
J Virol. 1981 Jun;38(3):996-1004. doi: 10.1128/JVI.38.3.996-1004.1981.
A 65,000-dalton (65K) antigen found in Raji cells by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis has been identified as an Epstein-Barr virus nuclear antigen (EBNA). This identification is based on the following evidence. The 65K antigen is detected in Raji cells but not in three Epstein-Barr virus (-) human B cell lines. It is not detected with EBNA (-) sera. The 65K antigen is found predominantly in the nucleus and co-elutes with EBNA during partial purification by DNA-Sepharose and Blue Dextran-Sepharose chromatography. Finally, the partially purified 65K antigen is an effective absorbant of EBNA antibody as measured in an anticomplement immunofluorescence assay. Antigens with molecular weights of 72, 70, and 73K have been detected in B95-8, P3HR-1, and Namalwa cells, respectively. These antigens are the likely homologues of the 65K Raji EBNA. In addition, an Epstein-Barr virus-associated, 81K DNA-binding antigen has been detected in both B95-8 and Raji cells.
通过荧光免疫电泳和放射免疫电泳在拉吉细胞中发现的一种65000道尔顿（65K）抗原已被鉴定为爱泼斯坦-巴尔病毒核抗原（EBNA）。这一鉴定基于以下证据。65K抗原在拉吉细胞中被检测到，但在三种爱泼斯坦-巴尔病毒（-）人B细胞系中未被检测到。用EBNA（-）血清检测不到它。65K抗原主要存在于细胞核中，并且在通过DNA-琼脂糖凝胶和蓝色葡聚糖-琼脂糖凝胶色谱进行部分纯化过程中与EBNA共洗脱。最后，在抗补体免疫荧光测定中，部分纯化的65K抗原是EBNA抗体的有效吸附剂。在B95-8、P3HR-1和纳马瓦细胞中分别检测到分子量为72K、70K和73K的抗原。这些抗原可能是65K拉吉EBNA的同源物。此外，在B95-8和拉吉细胞中都检测到了一种与爱泼斯坦-巴尔病毒相关的81K DNA结合抗原。
