Maca R D
Immunopharmacology. 1984 Oct;8(2):53-60. doi: 10.1016/0162-3109(84)90043-2.
The effects of cyclic nucleotides on the proliferation of cultured T-lymphocytes (CTC) stimulated by either phytohemagglutinin (PHA) or lectin-free interleukin-2 (IL-2) were studied. The addition of either N6,O2-dibutyryl cyclic AMP (DB-cAMP), aminophylline or isoproterenol to CTC cultures significantly suppressed the proliferation of CTC stimulated by either PHA or IL-2. This inhibitory effect was maximal when added at initiation of the assay; however, significant depression was still observed when added 24 h later. When DB-cAMP and aminophylline were added together, the inhibitory effects were additive. When DB-cGMP was added to the CTC cultures, inhibition of both PHA- and IL-2-stimulated cultures was also found, but the degree of activity was considerably less than for DB-cAMP or aminophylline. In contrast, when carbachol was added, no inhibition or modulation in proliferation was seen. Lastly, DB-cGMP was not found to antagonize the inhibitory effect of DB-cAMP, but instead to further increase the level of inhibition. In summary, these studies illustrate that cyclic nucleotides modulate the proliferation of IL-2-stimulated CTC as well as PHA-treated cells. This model system should provide an approach to study, in more depth, the mechanism by which cyclic nucleotides or their inducers can modulate the latter stages of the lymphocyte proliferative response, which is mediated by the lymphokine, IL-2.
研究了环核苷酸对由植物血凝素(PHA)或无凝集素白细胞介素-2(IL-2)刺激的培养T淋巴细胞(CTC)增殖的影响。向CTC培养物中添加N6,O2-二丁酰环磷酸腺苷(DB-cAMP)、氨茶碱或异丙肾上腺素可显著抑制由PHA或IL-2刺激的CTC增殖。在试验开始时添加这种抑制作用最大;然而,在24小时后添加仍观察到显著的抑制。当DB-cAMP和氨茶碱一起添加时,抑制作用是相加的。当向CTC培养物中添加DB-cGMP时,也发现对PHA和IL-2刺激的培养物均有抑制作用,但活性程度明显低于DB-cAMP或氨茶碱。相反,当添加卡巴胆碱时,未观察到对增殖的抑制或调节作用。最后,未发现DB-cGMP拮抗DB-cAMP的抑制作用,反而进一步增加了抑制水平。总之,这些研究表明环核苷酸可调节IL-2刺激的CTC以及PHA处理细胞的增殖。该模型系统应为更深入地研究环核苷酸或其诱导剂调节由淋巴因子IL-2介导的淋巴细胞增殖反应后期的机制提供一种方法。