von der Helm K, Deinhardt F
Leber Magen Darm. 1984 Nov;14(6):255-7.
The hepatitis A virus (HAV) belongs to the family of Picorna viruses/Enteroviridae. Its growing pattern in cell cultures deviates from the pattern found in other Picorna viruses: HAV does not have cytopathic effects and the replication rate of the virus in cell culture is lower than that one of comparable enteroviruses. In order to be able to understand the biochemistry and gene structure of this virus the genomic virus RNS was cloned molecularly as cDNS and the nucleotid sequence of the genom was partly clarified. The aim of our investigations is to characterize the neutralising antigen protein and the part of the gene coding for this protein, to express such a cloned part of the gene in bacteria, and to produce vaccine against HAV.
甲型肝炎病毒（HAV）属于微小核糖核酸病毒科/肠道病毒科。它在细胞培养中的生长模式与其他微小核糖核酸病毒不同：HAV没有细胞病变效应，且该病毒在细胞培养中的复制率低于类似肠道病毒。为了能够了解这种病毒的生物化学和基因结构，基因组病毒核糖核酸（RNS）被分子克隆为互补脱氧核糖核酸（cDNA），并且基因组的核苷酸序列已部分阐明。我们研究的目的是鉴定中和抗原蛋白以及编码该蛋白的基因部分，在细菌中表达该基因的这种克隆部分，并生产抗甲型肝炎病毒疫苗。
