甲型肝炎病毒cDNA在大肠杆菌中的表达：抗原性VP1重组蛋白
Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein.
作者信息
Ostermayr R, von der Helm K, Gauss-Müller V, Winnacker E L, Deinhardt F
机构信息
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Federal Republic of Germany.
出版信息
J Virol. 1987 Nov;61(11):3645-7. doi: 10.1128/JVI.61.11.3645-3647.1987.
The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.
甲型肝炎病毒（HAV）的基因组被逆转录成cDNA并进行分子克隆。编码携带主要HAV抗原的衣壳蛋白VP1的cDNA克隆被克隆到表达载体pUR290中，并在大肠杆菌中表达。重组融合蛋白在免疫印迹中与兔抗HAV血清发生反应，表明它具有HAV抗原性。
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