用抗根岸病毒单克隆抗体对黄病毒进行抗原分析。

Antigenic analysis of flaviviruses with monoclonal antibodies against Negishi virus.

作者信息

Furuta I, Takashima I, Arikawa J, Hashimoto N

出版信息

Microbiol Immunol. 1984;28(9):1023-30. doi: 10.1111/j.1348-0421.1984.tb00758.x.

DOI:10.1111/j.1348-0421.1984.tb00758.x

Abstract

Twelve monoclonal antibodies against Negishi virus were obtained and characterized by hemagglutination inhibition (HI) and neutralization (NT) tests using five flaviviruses isolated in the pan-Pacific region. The reaction pattern of the antibodies showed that Negishi virus was most closely related to Langat virus, followed by 3-Arch, JE and Apoi viruses in that order. Hemagglutinating (HA) antigen of the virus had distinct HI relating sites which were Negishi virus specific, tick borne encephalitis (TBE) virus complex specific and flavivirus cross-reactive. Monoclonal anti-Negishi antibodies cross-reactive to Japanese encephalitis (JE) virus in the HI test had neutralizing activity to JE virus but no activity to homologous Negishi virus.

摘要

获得了12种抗根岸病毒的单克隆抗体，并使用在泛太平洋地区分离出的5种黄病毒，通过血凝抑制（HI）和中和（NT）试验对其进行了表征。抗体的反应模式表明，根岸病毒与兰加特病毒关系最为密切，其次依次是3-Arch病毒、日本脑炎病毒和阿波伊病毒。该病毒的血凝（HA）抗原有不同的HI相关位点，这些位点是根岸病毒特异性的、蜱传脑炎（TBE）病毒复合体特异性的以及黄病毒交叉反应性的。在HI试验中与日本脑炎（JE）病毒交叉反应的抗根岸病毒单克隆抗体对JE病毒具有中和活性，但对同源根岸病毒无活性。