Kayser M, Klein H, Paasch I, Pilaski J, Blenk H, Heeg K
J Med Virol. 1985 Sep;17(1):35-45. doi: 10.1002/jmv.1890170106.
The antibody response against flaviviruses tick-borne encephalitis (TBE), Kyasanur Forest disease (KFD), Murray Valley encephalitis (MVE), West Nile fever (WNF), Japanese B encephalitis (JE), dengue 2 (DEN-2), and yellow fever (YF) was studied in humans after administration of an inactivated TBE virus vaccine. Individuals were either prevaccinated with 17D yellow fever (experimental group) or without any previous exposure to flaviviruses (control group). The appearance of serum titres of homologous and heterologous haemagglutination inhibition (HI) antibodies, heterotypic DEN-2 neutralizing antibodies, and TBE enzyme-linked immunosorbent assay (ELISA) antibodies were examined. Individuals prevaccinated with the 17D yellow fever developed an antibody pattern that contrasted with that of the control group. This pattern was characterized as follows: (1) Predominantly anti-TBE IgG antibodies appeared earlier and in higher titres than in the control group, (2) heterologous HI antibodies cross-reacting with the WN flavivirus subgroup preceded the appearance of homologous HI antibodies, (3) a broad spectrum HI response was observed against all flaviviruses tested, and (4) low titre heterotypic DEN-2 neutralizing antibodies were formed in about half of the cases. These observations are discussed in the context of cross-reactivity, cross-protection and virus infection enhancement.
在接种灭活蜱传脑炎（TBE）病毒疫苗后，研究了人类针对黄病毒属的蜱传脑炎（TBE）、卡萨努尔森林病（KFD）、墨累谷脑炎（MVE）、西尼罗热（WNF）、日本乙型脑炎（JE）、登革热2型（DEN-2）和黄热病（YF）的抗体反应。个体要么预先接种17D黄热病疫苗（实验组），要么之前未接触过任何黄病毒（对照组）。检测了同源和异源血凝抑制（HI）抗体、异型DEN-2中和抗体以及TBE酶联免疫吸附测定（ELISA）抗体的血清滴度出现情况。预先接种17D黄热病疫苗的个体产生的抗体模式与对照组不同。这种模式的特征如下：（1）主要的抗TBE IgG抗体出现得比对照组更早且滴度更高，（2）与西尼罗病毒亚组发生交叉反应的异源HI抗体先于同源HI抗体出现，（3）观察到针对所有测试黄病毒的广谱HI反应，（4）约一半病例中形成了低滴度的异型DEN-2中和抗体。在交叉反应性、交叉保护和病毒感染增强的背景下讨论了这些观察结果。
