Macartney L, McCandlish I A, Thompson H, Cornwell H J
Vet Rec. 1984 Nov 24;115(21):533-7. doi: 10.1136/vr.115.21.533.
A group of 10-week-old puppies was orally inoculated with canine parvovirus of faecal origin. Scanning electron microscopy was used to study and compare the surface topography in both control and inoculated animals. In control dogs the villi were tall and finger-like in shape and numerous irregular transverse circumferential grooves were present on the surface. At higher magnification, the outlines of individual epithelial cells and depressions, interpreted as goblet cells, could be discerned. In the inoculated dogs, scanning electron microscopy changes were first seen at six days after inoculation. The small intestinal mucosa was covered by a thick layer of mucus. The underlying villi were stunted and had lost their surface features. In some instances there was loss of the luminal epithelium, exposing the lamina propria. In addition, there was dilation of the circumvillar basins and the crypt mouths. There appeared to be regenerative changes by day 7 after inoculation. The surface of the small intestinal mucosa was still covered by a thick layer of mucus. Where villi could be discerned, they were short and pointed and transverse grooves could be seen on their surface. There was some hypertrophy of the intervillus ridges. The changes in the surface topography of the small intestinal mucosa following canine parvovirus infection are compared to those seen in enteric infections in other species and the similarity of the lesion to that seen following sublethal irradiation is discussed.
一组10周龄的幼犬经口接种粪便来源的犬细小病毒。利用扫描电子显微镜研究并比较了对照动物和接种动物的表面形貌。在对照犬中，绒毛高且呈指状，表面有许多不规则的横向圆周沟。在更高放大倍数下，可以辨别出单个上皮细胞的轮廓以及被认为是杯状细胞的凹陷。在接种犬中，接种后6天首次观察到扫描电子显微镜下的变化。小肠黏膜被一层厚厚的黏液覆盖。其下的绒毛发育不良，表面特征消失。在某些情况下，管腔上皮缺失，固有层暴露。此外，绒毛周围隐窝和隐窝口扩张。接种后第7天似乎出现了再生变化。小肠黏膜表面仍被一层厚厚的黏液覆盖。在能辨别出绒毛的地方，它们短而尖，表面可见横向沟。绒毛间嵴有一些肥大。将犬细小病毒感染后小肠黏膜表面形貌的变化与其他物种肠道感染时的变化进行了比较，并讨论了该病变与亚致死剂量辐射后所见病变的相似性。
