Begum K D, Mottola H A
Anal Biochem. 1984 Oct;142(1):1-6. doi: 10.1016/0003-2697(84)90508-6.
The design and performance of an enzyme reactor (enzyme electrode) which features (i) incorporating nylon shavings onto which an enzyme is covalently bonded, (ii) a flat-surface combination pH electrode for proton monitoring, and (iii) a body providing an injection port for sample injection and washing and stirring capabilities is described. The reactor configuration described here offers good diffusional and partition characteristics which result in relatively fast response, good stability, simplicity of operation, low sample and reagent consumption, and adaptability to flow systems. Application to the determination of urea in standards and physiological salt solutions is demonstrated by use of immobilized urease (EC 3.5.1.5).
本文描述了一种酶反应器（酶电极）的设计与性能，其特点包括：（i）将共价结合有酶的尼龙碎屑纳入其中；（ii）用于质子监测的平面组合pH电极；（iii）具有样品注入端口以及具备清洗和搅拌能力的主体。这里所描述的反应器结构具有良好的扩散和分配特性，从而带来相对快速的响应、良好的稳定性、操作简便、低样品和试剂消耗以及对流动系统的适应性。通过使用固定化脲酶（EC 3.5.1.5）证明了其在标准溶液和生理盐溶液中尿素测定的应用。
