Sundaram P V, Igloi M P, Wassermann R, Hinsch W, Knoke K J
Clin Chem. 1978 Feb;24(2):234-9.
A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried out routinely with the immobilized enzyme nylon tube reactor give reliable and reproducible results with high precision and low cost. The reactors are stable to intermittent or continued use for at least four months or for 2000 tests. A method is described in which differential colorimetry is used for determining citrulline in blood and which makes use of the immobilized urease, albeit indirectly.
本文描述了一种用于常规测定尿素的连续流动临床分析仪，该分析仪使用固定化酶尼龙管反应器作为流通系统（Technicon AutoAnalyzer I）的一部分。将使用固定化脲酶进行的血尿素分析结果与用二乙酰一肟法和脲酶溶液法进行的测定结果进行了比较。使用固定化酶尼龙管反应器进行的常规临床试验给出了可靠且可重复的结果，具有高精度和低成本。这些反应器对于间歇或连续使用至少四个月或进行2000次测试都很稳定。本文还描述了一种方法，该方法使用差示比色法间接利用固定化脲酶来测定血液中的瓜氨酸。
