通过四唑还原法测定硫氰酸酶活性。

Determination of rhodanese activity by tetrazolium reduction.

作者信息

Cannella C, Berni R, Ricci G

出版信息

Anal Biochem. 1984 Oct;142(1):159-62. doi: 10.1016/0003-2697(84)90531-1.

Abstract

A colorimetric method for the assay of rhodanese activity based on the continuous determination of the sulfite product is described. 5-Ethylphenazinium ethyl sulfate is used as the intermediate electron carrier between sulfite and nitroblue tetrazolium to produce the colored reduced species. The present method is more sensitive than the usual procedure based on the colorimetric determination of thiocyanate. Furthermore, the color developed by nitroblue tetrazolium reduction affords a straightforward means to locate rhodanese activity in polyacrylamide gels.

摘要

本文描述了一种基于连续测定亚硫酸盐产物来检测硫氰酸酶活性的比色法。5-乙基硫酸吩嗪鎓用作亚硫酸盐和硝基蓝四唑之间的中间电子载体，以产生显色的还原产物。本方法比基于硫氰酸盐比色测定的常规方法更灵敏。此外，通过硝基蓝四唑还原产生的颜色为在聚丙烯酰胺凝胶中定位硫氰酸酶活性提供了一种直接的方法。