Dickinson A C, DeJordy J O, Boutin M G, Teres D
Biochem Biophys Res Commun. 1984 Dec 14;125(2):584-91. doi: 10.1016/0006-291x(84)90579-5.
In vitro ESR studies of doxorubicin and 4'-deoxydoxorubicin have been conducted using the spin traps PBN and DMPO. Solutions prepared in the presence of atmospheric oxygen at pH = 12 exhibited a typical semiquinone signal preceded by a short-lived signal attributed to perhydroxyl radical, HO2. At physiological pH the perhydroxyl signal was observed in the doxorubicin solution but not in 4'-deoxydoxorubicin. In the absence of oxygen, at pH = 7.5, neither drug exhibited a signal. A relationship is proposed between the perhydroxyl radical and the cardiotoxicity observed in doxorubicin but not in 4'-deoxydoxorubicin.
已使用自旋捕捉剂PBN和DMPO对阿霉素和4'-脱氧阿霉素进行了体外电子自旋共振（ESR）研究。在pH = 12的大气氧存在下制备的溶液表现出典型的半醌信号，之前有一个归因于过羟基自由基HO2的短暂信号。在生理pH值下，在阿霉素溶液中观察到了过羟基信号，但在4'-脱氧阿霉素中未观察到。在无氧条件下，pH = 7.5时，两种药物均未显示信号。有人提出过羟基自由基与阿霉素中观察到的心脏毒性之间存在关联，而在4'-脱氧阿霉素中未观察到这种关联。
