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[鼠疫耶尔森菌EV 76 C dam和dcm的腺嘌呤与胞嘧啶DNA甲基化酶的特异性识别位点与大肠杆菌甲基化酶的比较]

[Comparison of specific recognition sites of adenine and cytosine DNA-methylase of Yersinia Pestis EV 76 C dam and dcm by Escherichia coli methylases].

作者信息

Demidova G V, Goncharov E K, Tynianova V I

出版信息

Biokhimiia. 1984 Oct;49(10):1594-7.

PMID:6097301
Abstract

Using enzymatic modelling of in vitro methylation of chromosome DNAs from Yersinia pestis EV 76, E. coli 834 and E. coli C600 RII by DNA methylases of Eco RII and Eco dam as well as of DNA hydrolysis of plasmid pBR 322 from the cells of Y. pestis EV 76, E. coli C600 and E. coli 834 by restrictases of Eco RII and Cfu I, it was found that cytosine DNA methylase from plague bacteria does not correspond to the type of RII methylases of E. coli. Adenyl DNA methylase is related to E. coli methylases type dam and modifies adenine in the nucleotide sequence of GATC.

摘要

利用鼠疫耶尔森菌EV 76、大肠杆菌834和大肠杆菌C600 RII的染色体DNA经Eco RII和Eco dam的DNA甲基化酶进行体外甲基化的酶促模拟,以及鼠疫耶尔森菌EV 76、大肠杆菌C600和大肠杆菌834细胞中的质粒pBR 322经Eco RII和Cfu I的限制酶进行DNA水解,发现来自鼠疫细菌的胞嘧啶DNA甲基化酶与大肠杆菌的RII型甲基化酶类型不对应。腺苷DNA甲基化酶与大肠杆菌的dam型甲基化酶相关,并修饰GATC核苷酸序列中的腺嘌呤。

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