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Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.位点特异性修饰对限制性核酸内切酶和DNA修饰甲基转移酶的影响。
Nucleic Acids Res. 1994 Sep;22(17):3640-59. doi: 10.1093/nar/22.17.3640.
3
The sequence of IS4.IS4的序列。
Mol Gen Genet. 1981;181(2):169-75. doi: 10.1007/BF00268423.
4
Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化对DNA修饰甲基转移酶和限制性内切核酸酶的影响。
Nucleic Acids Res. 1989;17 Suppl(Suppl):r389-415. doi: 10.1093/nar/17.suppl.r389.
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Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化：对DNA修饰甲基转移酶和限制性内切酶的影响。
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6
Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.位点特异性甲基化对DNA修饰甲基转移酶和限制性内切酶的影响。
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The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. X. Bacteriophage T3-induced S-adenosylmethionine cleavage.核糖核酸和脱氧核糖核酸的酶促甲基化作用。X. 噬菌体T3诱导的S-腺苷甲硫氨酸裂解
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大肠杆菌SK中胞嘧啶DNA甲基化酶识别位点的测定。

Determination of the recognition sites of cytosine DNA-methylases from Escherichia coli SK.

作者信息

Nikolskaya I I, Lopatina N G, Anikeicheva N V, Debov S S

出版信息

Nucleic Acids Res. 1979 Sep 25;7(2):517-28. doi: 10.1093/nar/7.2.517.

DOI:10.1093/nar/7.2.517
PMID:386287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328033/
Abstract

Two different cytosine DNA-methylases, NI and GII, are present in Escherichia coli SK. The GII methylase recognizes the five-member symmetric sequence: 5'...NpCpCpApGpGpN...3'. This sequence is identical with the recognition site of the hsp II type determined by RII plasmid but, in contrast to RII methylase, the GII enzyme methylates cytosine located on the 5' side of the site. By analogy with the isoshizomery of the restricting endonucleases, RII and GII DNA methylaeses may be called isomethymers which recognize the same site but methylate different bases. Since the phage of the SK and hsp II phenotypes is effectively restricted in respective cells it may be assumed that the isomethymeric modification does not provide any protection against the corresponding restrictases. NI methylase recognizes the five-member symmetric site which represents an inverted sequence of the GII site: 5'...NpGpGpApCpCpN...3'. In this case cytosine at the 3'-end of the recognition site is methylated.

摘要

在大肠杆菌SK中存在两种不同的胞嘧啶DNA甲基化酶，即NI和GII。GII甲基化酶识别五元对称序列：5'...NpCpCpApGpGpN...3'。该序列与由RII质粒确定的hsp II型识别位点相同，但与RII甲基化酶不同的是，GII酶使位于该位点5'侧的胞嘧啶甲基化。类似于限制性内切酶的同裂酶，RII和GII DNA甲基化酶可被称为识别相同位点但甲基化不同碱基的同甲基化酶。由于具有SK和hsp II表型的噬菌体在各自的细胞中受到有效限制，因此可以推测同甲基化修饰不能提供针对相应限制酶的任何保护。NI甲基化酶识别一个五元对称位点，该位点是GII位点的反向序列：5'...NpGpGpApCpCpN...3'。在这种情况下，识别位点3'端的胞嘧啶被甲基化。