Sobolev A S, Rosenkranz A A, Kazarov A R
Biosci Rep. 1984 Oct;4(10):897-902. doi: 10.1007/BF01138172.
Extraction of the 'fluid' lipid fraction from rat reticulocyte plasma membranes by phospholipase/BSA treatment leads to a reduction in the percentage of beta-adrenoreceptors complexed with the regulatory N-proteins and to a decrease of adenylate cyclase stimulation with a beta-agonist. Analysis of the data obtained on the basis of the percolation theory suggests that proteins of the adenylate cyclase complex move along the whole membrane.
用磷脂酶/牛血清白蛋白处理从大鼠网织红细胞质膜中提取“流体”脂质部分,会导致与调节性N蛋白结合的β-肾上腺素能受体百分比降低,并使β-激动剂刺激的腺苷酸环化酶活性下降。基于渗流理论对所得数据的分析表明,腺苷酸环化酶复合物的蛋白质在整个膜上移动。