Feix J B, Khatri B, McQuillen M P, Koethe S M
Immunol Commun. 1984;13(5):465-74. doi: 10.3109/08820138409033893.
A spin-membrane immunoassay has been employed to examine the immune reactivity of whole serum from patients with chronic-progressive multiple sclerosis (CPMS) against liposomes containing ganglioside GM1. Exposure to serum resulted in complement-mediated lysis of the GM1-liposomes. No lysis occurred with liposomes devoid of ganglioside. The mean (+/- S.E.M.) lysis values were 52.6 (+/- 9.8)% for fifteen CPMS patients and 32.9 (+/- 7.2)% for nine controls. The difference between the means was highly significant (student's t-test, P less than 0.0001), indicating increased anti-ganglioside immunity in patients with CPMS.
一种自旋膜免疫测定法已被用于检测慢性进行性多发性硬化症（CPMS）患者的全血清对含有神经节苷脂GM1的脂质体的免疫反应性。血清作用导致GM1脂质体发生补体介导的溶解。不含神经节苷脂的脂质体未发生溶解。15名CPMS患者的平均（±标准误）溶解值为52.6（±9.8）%，9名对照者的平均溶解值为32.9（±7.2）%。平均值之间的差异具有高度显著性（学生t检验，P<0.0001），表明CPMS患者的抗神经节苷脂免疫增强。
