Monboisse J C, Poulin G, Braquet P, Randoux A, Ferradini C, Borel J P
Int J Tissue React. 1984;6(5):385-90.
Fibrils of collagen reconstituted in vitro by dialysis against sodium formate are exposed to free oxy radicals generated by three different systems: (i) xanthine oxidase + hypoxanthine, (ii) gamma-rays originating from a cobalt bomb; (iii) pulse radiolysis in a particle accelerator. A degradation of the collagen fibres is demonstrated by determination of the amount of hydroxyproline-containing peptides in the supernatant after incubation. Types I and III collagen are sensitive to the effect, whereas type V collagen is not. The effect persists when collagen is specially delipidated.
通过对甲酸钠进行透析在体外重构的胶原纤维，暴露于由三种不同系统产生的游离氧自由基中：（i）黄嘌呤氧化酶+次黄嘌呤；（ii）钴源γ射线；（iii）粒子加速器中的脉冲辐解。孵育后，通过测定上清液中含羟脯氨酸肽的量来证明胶原纤维的降解。I型和III型胶原对这种作用敏感，而V型胶原则不敏感。当胶原经过特殊脱脂处理后，这种作用仍然存在。
