Hlubinová K, Prachar J, Vrbenská A, Matoska J, Simkovic D
Neoplasma. 1984;31(6):631-40.
Endogenous retroviruses are not in the majority of cases the cause of any neoplasia, except for the laboratory conditions. As far as they might serve for the evolution of pathogenic retroviruses more attention should have been paid to them. In this paper we introduce some approaches to the purification of rat endogenous retroviruses to such a degree of purity that enabled satisfactory SDS-PAGE analysis of its structural proteins. Purities of samples obtained by usual purification methods, long-term isopycnic centrifugation at a high gravity force and velocity centrifugation are compared. Protein profile of rat endogenous virus in SDS-PAGE is compared with the ones of other retroviruses. For the first time the evidence was obtained for the striking similarity between electrophoretic protein profile of rat endogenous virus WERC and feline leukemia virus. The major structural proteins of rat endogenous retrovirus and feline leukemia virus cannot be distinguished even when resolution long gradient PAGE had been employed. The accordance of electrophoretic mobilities of major structural proteins in SDS-PAGE can indicate the relatedness of retroviruses.
除了在实验室条件下，内源性逆转录病毒在大多数情况下并非任何肿瘤形成的原因。就它们可能有助于致病性逆转录病毒的进化而言，本应给予它们更多关注。在本文中，我们介绍了一些纯化大鼠内源性逆转录病毒的方法，使其纯度达到能够对其结构蛋白进行令人满意的SDS-PAGE分析的程度。比较了通过常规纯化方法、在高重力下进行长期等密度离心和速度离心所获得样品的纯度。将大鼠内源性病毒在SDS-PAGE中的蛋白质谱与其他逆转录病毒的蛋白质谱进行了比较。首次获得证据表明大鼠内源性病毒WERC的电泳蛋白质谱与猫白血病病毒的电泳蛋白质谱具有惊人的相似性。即使采用分辨率长梯度PAGE，大鼠内源性逆转录病毒和猫白血病病毒的主要结构蛋白也无法区分。SDS-PAGE中主要结构蛋白的电泳迁移率一致可以表明逆转录病毒之间的相关性。
