超螺旋pBR322 DNA的臭氧分解导致链断裂形成开环DNA。
Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.
作者信息
Sawadaishi K, Miura K, Ohtsuka E, Ueda T, Ishizaki K, Shinriki N
出版信息
Nucleic Acids Res. 1985 Oct 25;13(20):7183-94. doi: 10.1093/nar/13.20.7183.
Abstract
Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis in the absence of salt caused single strand cleavage at specific sites.
摘要
用臭氧处理超螺旋pBR322 DNA导致闭环DNA转变为开环DNA。对所得开环DNA的限制性分析表明，在无盐情况下的臭氧分解会在特定位点造成单链断裂。
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