Kiel J L, Erwin D N
Physiol Chem Phys Med NMR. 1984;16(4):317-23.
The influence of microwave radiation (2450 MHz, 3,333 pulses per second, duty factor of 0.02, and average specific absorption rate of 0.4 W/kg) on spontaneous hemolysis of human erythrocytes was examined. Cells were exposed to microwave radiation for 20 minutes at 37 degrees, 42 degrees, or 48 degrees C. Some of these cells were sensitized to oxidative damage by treatment with 1-chloro-2,4-dinitrobenzene (CDNB) and/or by coating with wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate. Microwave radiation significantly decreased spontaneous hemolysis of untreated cells at 42 degrees C but had no effect at 37 degrees or 48 degrees C. Microwave exposure significantly enhanced a CDNB membrane stabilizing effect at 42 degrees C but had no effect at 37 or 48 degrees C. At 42 degrees C, microwave exposure increased hemolysis of WGA-HRP coated cells. Cells treated with both WGA-HRP and CDNB showed no change in fragility at 42 degrees C and increased fragility at 48 degrees C without a microwave effect. The microwave effects observed appear to involve perturbation of the thermal threshold for oxidative hyperthermic hemolysis.
研究了微波辐射（2450兆赫，每秒3333个脉冲，占空比0.02，平均比吸收率0.4瓦/千克）对人红细胞自发溶血的影响。将细胞在37℃、42℃或48℃下暴露于微波辐射20分钟。其中一些细胞通过用1-氯-2,4-二硝基苯（CDNB）处理和/或用麦胚凝集素-辣根过氧化物酶（WGA-HRP）偶联物包被而对氧化损伤敏感。微波辐射显著降低了42℃下未处理细胞的自发溶血，但在37℃或48℃下没有影响。微波暴露在42℃时显著增强了CDNB的膜稳定作用，但在37℃或48℃时没有影响。在42℃时，微波暴露增加了WGA-HRP包被细胞的溶血。用WGA-HRP和CDNB处理的细胞在42℃时脆性没有变化，在48℃时脆性增加且没有微波效应。观察到的微波效应似乎涉及氧化热溶血热阈值的扰动。
