Karlsson J A, Persson C G
Acta Physiol Scand. 1984 Dec;122(4):589-98. doi: 10.1111/j.1748-1716.1984.tb07548.x.
Neurogenic responses in isolated electrical field stimulated guinea-pig tracheas include a prominent relaxation that is only partially inhibited by propranolol. The possibility that either purine derivatives or VIP could be a non-adrenergic inhibitory neurotransmitter was examined. Relaxant characteristics of adenosine, ATP, adenine and VIP in tracheas with different degrees of contraction were compared to those of maximal non-adrenergic nerve stimulation. Spontaneous tone and carbachol (5.5 microM and 55 microM)-contracted tracheas were inhibited 77.5 +/- 5.0% (n = 9), 24.7 +/- 2.8% (n = 11) and 14.5 +/- 5.0% (n = 10), respectively, by electrical nerve stimulation. At the three states of contraction exogenous VIP, but not adenosine, ATP, or adenine, produced relaxations similar to those of electrical nerve stimulation. In carbachol-contracted tracheas with maximally effective concentrations present of adenosine (3 mM), adenine (3 mM) or VIP (23.3 microM), the non-adrenergic neurogenic inhibitory response remained intact. The neurogenic inhibitory response was also unaffected by the presence of theophylline and indomethacin which blocked relaxatory effects of adenosine and ATP, respectively. The results suggests that neither the purine derivatives, nor VIP are involved in the non-adrenergic, neurogenic inhibition in the guinea-pig trachea.
在分离的电场刺激豚鼠气管中，神经源性反应包括显著的舒张，这种舒张仅被普萘洛尔部分抑制。研究了嘌呤衍生物或血管活性肠肽（VIP）是否可能是一种非肾上腺素能抑制性神经递质。将腺苷、三磷酸腺苷（ATP）、腺嘌呤和VIP在不同收缩程度气管中的舒张特性与最大非肾上腺素能神经刺激的舒张特性进行了比较。电神经刺激分别使自发张力以及由卡巴胆碱（5.5微摩尔和55微摩尔）收缩的气管抑制了77.5±5.0%（n = 9）、24.7±2.8%（n = 11）和14.5±5.0%（n = 10）。在三种收缩状态下，外源性VIP而非腺苷、ATP或腺嘌呤产生了与电神经刺激相似的舒张。在存在最大有效浓度的腺苷（3毫摩尔）、腺嘌呤（3毫摩尔）或VIP（23.3微摩尔）的卡巴胆碱收缩的气管中，非肾上腺素能神经源性抑制反应保持完整。茶碱和吲哚美辛分别阻断了腺苷和ATP的舒张作用，但神经源性抑制反应不受其存在的影响。结果表明，嘌呤衍生物和VIP均不参与豚鼠气管中的非肾上腺素能神经源性抑制。
