Cloix J F, Henning G, Crabos M, Delva P, Meyer P
Arch Mal Coeur Vaiss. 1984 Oct;77(11):1251-5.
Semi-purified dog kidney Na+-K+-ATPase was cross-linked with ovalbumin. This immobilized enzyme was able to hydrolyse ATP and this hydrolysis was ouabain-sensitive. It was then used in batch wise affinity chromatography for the detection of endogenous Na+-K+-ATPase inhibitor in human plasma and urine. Ammonium acetate 1 mM washed off the endogenous Na+-K+-ATPase inhibitor from the immobilized enzyme. The inhibitory activity of the eluate from hypertensive plasma was significantly higher (p less than 0.0025, n = 6) than that of normotensive plasma. Similar results were obtained (n = 3) from human urine eluates during salt loading as compared to control urine.
半纯化的犬肾钠钾ATP酶与卵清蛋白交联。这种固定化酶能够水解ATP，且这种水解对哇巴因敏感。然后将其用于分批亲和色谱法，以检测人血浆和尿液中的内源性钠钾ATP酶抑制剂。1 mM醋酸铵可从固定化酶上洗脱内源性钠钾ATP酶抑制剂。高血压患者血浆洗脱液的抑制活性显著高于正常血压患者血浆（p<0.0025，n = 6）。与对照尿液相比，在盐负荷期间从人尿液洗脱液中也获得了类似结果（n = 3）。
