Xie Z X, Stone H D
U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Avian Dis. 1990 Jan-Mar;34(1):154-62.
Inactivated Newcastle disease virus (NDV), avian influenza virus (AIV), and infectious bronchitis virus (IBV) antigens were evaluated for immunological efficacy in monovalent and polyvalent vaccines. Vaccinated broilers were bled for hemagglutination-inhibition (HI) tests at 1- or 2-week intervals. Half of the chickens were challenged with the Largo isolate of velogenic viscerotropic (VV) NDV at 8 weeks post-vaccination, and the remainder were challenged with the Massachusetts 41 strain IBV at 9 weeks post-vaccination. Newcastle disease HI titers were reduced significantly (P less than 0.05) from those of monovalent control vaccine groups when IBV antigen was emulsified in mixtures with low (1-3x) concentrated NDV or NDV and AIV antigens. Avian influenza HI titers were significantly (P less than 0.05) lower than those of the control monovalent groups when highly concentrated NDV was part of the polyvalent vaccine. Infectious bronchitis HI titers were higher than those of control monovalent groups in 13 of 15 vaccine groups when IBV antigen was in polyvalent formulations. VV NDV challenge killed all non-NDV vaccinates and induced increased HI titers in NDV vaccinates but no morbidity or mortality. Sixty of 80 IBV vaccinates experienced a fourfold or greater HI titer increase following challenge. All non-IBV vaccinates seroconverted at 1 week post-challenge.
对灭活的新城疫病毒（NDV）、禽流感病毒（AIV）和传染性支气管炎病毒（IBV）抗原在单价和多价疫苗中的免疫效果进行了评估。每隔1周或2周对接种疫苗的肉鸡采血进行血凝抑制（HI）试验。一半的鸡在接种疫苗后8周用速发型嗜内脏型（VV）NDV的拉戈毒株进行攻毒，其余的鸡在接种疫苗后9周用马萨诸塞41株IBV进行攻毒。当IBV抗原与低浓度（1-3倍）浓缩的NDV或NDV和AIV抗原混合乳化时，新城疫HI效价与单价对照疫苗组相比显著降低（P小于0.05）。当高浓度的NDV作为多价疫苗的一部分时，禽流感HI效价显著低于对照单价组（P小于0.05）。当IBV抗原处于多价制剂中时，15个疫苗组中有13个组的传染性支气管炎HI效价高于对照单价组。VV NDV攻毒导致所有未接种NDV疫苗的鸡死亡，并使接种NDV疫苗的鸡的HI效价升高，但未引起发病或死亡。80只接种IBV疫苗的鸡中有60只在攻毒后HI效价升高了四倍或更高。所有未接种IBV疫苗的鸡在攻毒后1周出现血清转化。
