在口服免疫小鼠中引发 IgA 单克隆抗体的 3 型脊髓灰质炎病毒的抗原位点。

Antigenic sites of poliovirus type 3 eliciting IgA monoclonal antibodies in orally immunized mice.

作者信息

Buttinelli G, Ruggeri F M, Marturano J, Novello F, Donati V, Fiore L

机构信息

Laboratory of Virology, Laboratory of Ultrastructure, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.

出版信息

Virology. 2001 Mar 15;281(2):265-71. doi: 10.1006/viro.2000.0786.

DOI:10.1006/viro.2000.0786

PMID:11277698

Abstract

A panel of neutralizing IgA monoclonal antibodies was produced from mice orally inoculated with poliovirus type 3 Sabin and cholera toxin as adjuvant. Low levels of neutralizing antibodies were elicited in mice after several boosts, but only in the presence of cholera toxin. Characterization of IgA MAbs by neutralization-escape virus mutants showed that all but one neutralizing MAbs against type 3 poliovirus were directed to antigenic site N-AgIII, which was previously found by us to be the major target of mucosal immune response to Sabin 1 in the mouse. Our data indicate that residue 236 of VP3, not previously reported, is also involved in forming site N-AgIII in addition to formerly described VP3 (aa 58-59) and VP1 (aa 286-290) residues. Unlike poliovirus type 1 IgA MAbs, all IgA MAbs herein described neutralized the wild-type parental poliovirus.

摘要

用3型脊髓灰质炎病毒萨宾株口服接种小鼠，并以霍乱毒素作为佐剂，制备了一组中和性IgA单克隆抗体。经过几次加强免疫后，小鼠体内诱导产生了低水平的中和抗体，但仅在霍乱毒素存在的情况下。通过中和逃逸病毒突变体对IgA单克隆抗体进行表征，结果显示，除一种针对3型脊髓灰质炎病毒的中和单克隆抗体外，其余所有中和单克隆抗体均靶向抗原位点N-AgIII，我们之前在小鼠中发现该位点是对萨宾1型疫苗黏膜免疫反应的主要靶点。我们的数据表明，除了先前描述的VP3（第58-59位氨基酸）和VP1（第286-290位氨基酸）残基外，之前未报道的VP3第236位残基也参与形成位点N-AgIII。与1型脊髓灰质炎病毒IgA单克隆抗体不同，本文所述的所有IgA单克隆抗体均能中和野生型亲代脊髓灰质炎病毒。