Friedman R, Gelfand T, Weiss D W, Doljanski F
Int J Tissue React. 1984;6(4):291-301.
Deposition of fibronectin (FN) was studied by indirect immunofluorescent staining, employing monospecific rabbit anti-human FN antibody, in the following 3 systems: cultures of cells derived from Rous sarcomas of chickens and of normal chick fibroblasts; frozen sections of chicken Rous sarcomas; and frozen sections of mouse and human mammary tissue, normal and neoplastic. The normal fibroblasts produced a well-oriented, delicate, fibrillar FN network. The sarcoma cells in culture also formed a FN-positive matrix, but one very different in appearance, consisting of coarse, unoriented strands. FN was also present abundantly between the cells of the sarcomas in vivo. In normal mammary tissue, FN was localized predominantly in the basal lamina region, well delineated from the surrounding FN-reactive stroma. The epithelial cells were generally, but not always, FN-negative. In apparently normal tissue areas of the breasts of mammary carcinoma patients, epithelial cells with highly reactive cell peripheries were frequently seen; the stroma surrounding the positive cells was often poor in FN and the basal lamina region lacked the substance. Mammary carcinoma cells were almost always negative, but it was characteristic of these tumours that the surrounding stroma displayed FN richly.
采用单特异性兔抗人纤连蛋白（FN）抗体，通过间接免疫荧光染色，在以下3个系统中研究了纤连蛋白（FN）的沉积情况：源自鸡劳斯肉瘤和正常鸡成纤维细胞的细胞培养物；鸡劳斯肉瘤的冰冻切片；以及正常和肿瘤性的小鼠及人乳腺组织的冰冻切片。正常成纤维细胞产生了一个排列良好、精细的纤维状FN网络。培养中的肉瘤细胞也形成了一个FN阳性基质，但外观非常不同，由粗大、无定向的条索组成。在体内肉瘤细胞之间也大量存在FN。在正常乳腺组织中，FN主要定位于基膜区域，与周围FN反应性基质界限清晰。上皮细胞通常但并非总是FN阴性。在乳腺癌患者乳房明显正常的组织区域，经常可见细胞周边反应性高的上皮细胞；阳性细胞周围的基质通常FN含量低，基膜区域缺乏该物质。乳腺癌细胞几乎总是阴性，但这些肿瘤的特征是周围基质富含FN。
