Tashima Y, Mizunuma H, Shima H, Kobayashi R, Hakeda Y, Maeda N, Kumegawa M
J Biochem. 1984 Nov;96(5):1619-24. doi: 10.1093/oxfordjournals.jbchem.a134992.
Glucocorticoids significantly affected the developmental appearance of fructose-1,6-bisphosphatase [EC 3.1.3.11] and glucose-6-phosphatase [EC 3.1.3.9] in fetal mouse liver. In fragments of 15- or 16-day-old fetal livers maintained in organ culture in the absence of serum, induction of the bisphosphatase by dibutyryl cyclic AMP was repressed completely when the tissue was treated with 10(-7)M dexamethasone for 24 h during the second day of culture. The induction of the glucose phosphatase was greatly stimulated after a lag of 1 to 2 days. The glucocorticoid action continued over a period of 2 days even though the steroid had been washed out. The dose response curve of hydrocortisone with the half-maximally effective concentration of roughly 2 X 10(-8)M is in the physiological range. The corticoid action was specific for glucocorticoids, and aldosterone or progesterone was ineffective. When the tissue was cultured for 4 days before addition of dexamethasone, the bisphosphatase induction became insensitive to the steroid. Glucose-6-phosphatase induction, however, remained sensitive, but the long latent period required for the appearance of the hormone action disappeared. These results indicate the involvement of glucocorticoids in the developmental appearance of glucose-6-phosphatase in fetal liver.
糖皮质激素显著影响胎鼠肝脏中果糖-1,6-二磷酸酶[EC 3.1.3.11]和葡萄糖-6-磷酸酶[EC 3.1.3.9]的发育表现。在无血清条件下进行器官培养的15或16日龄胎肝组织块中，当在培养的第二天用10(-7)M地塞米松处理组织24小时时，二丁酰环磷酸腺苷对双磷酸酶的诱导作用被完全抑制。葡萄糖磷酸酶的诱导在滞后1至2天后受到极大刺激。即使类固醇已被洗脱，糖皮质激素的作用仍持续2天。氢化可的松的剂量反应曲线，其半数有效浓度约为2×10(-8)M，处于生理范围内。皮质激素的作用对糖皮质激素具有特异性，醛固酮或孕酮无效。当地塞米松添加前组织培养4天时，双磷酸酶的诱导对该类固醇变得不敏感。然而，葡萄糖-6-磷酸酶的诱导仍然敏感，但激素作用出现所需的长潜伏期消失了。这些结果表明糖皮质激素参与了胎肝中葡萄糖-6-磷酸酶的发育表现。
