Takeuchi A, Okano T, Ayame M, Yoshikawa H, Teraoka S, Murakami Y, Kobayashi T
J Nutr Sci Vitaminol (Tokyo). 1984 Oct;30(5):421-30. doi: 10.3177/jnsv.30.421.
Identification and determination of vitamin D3 (or D2) and 25-OH-D3 in fish liver oils and eel body oils were carried out. By co-chromatography on HPLC, UV spectra and/or GC-MS, vitamin D3 was identified in naturally occurring fish liver oils and eel body oils, whereas a drop of fish liver oil contained supplemented vitamin D2. 25-OH-D3 was identified only in skipjack liver oil. The HPLC method proposed in a previous report (Takeuchi, A. et al. (1984): J. Nutr. Sci. Vitaminol., 30, 11-25) was confirmed to also be useful for determination of vitamin D3 (or D2) in fish liver oils and eel body oils. The assayed values of vitamin D3 in skipjack and tuna liver oils were 57,760 and 16,200 IU/g, respectively, which were much higher than those in cod and pollack liver oils. The assayed values of vitamin D3 in eel body oils were very low (16-43 IU/g) and showed no appreciable change despite differences in the farming conditions. Determination of 25-OH-D3 in skipjack oil was performed by using HPLC, and the assayed value was 1.8 micrograms/g. This was about 1/800 lower than that of vitamin D3.
对鱼肝油和鳗鱼体油中的维生素D3（或D2）以及25-羟基-D3进行了鉴定和测定。通过高效液相色谱（HPLC）、紫外光谱和/或气相色谱-质谱联用的共色谱法，在天然的鱼肝油和鳗鱼体油中鉴定出了维生素D3，而一滴鱼肝油中含有添加的维生素D2。仅在鲣鱼肝脏油中鉴定出了25-羟基-D3。先前报告（竹内，A.等人（1984年）：《营养科学与维生素学杂志》，30，11-25）中提出的HPLC方法被证实也可用于测定鱼肝油和鳗鱼体油中的维生素D3（或D2）。鲣鱼和金枪鱼肝脏油中维生素D3的测定值分别为57,760和16,200国际单位/克，远高于鳕鱼和狭鳕鱼肝脏油中的测定值。鳗鱼体油中维生素D3的测定值非常低（16 - 43国际单位/克），并且尽管养殖条件不同，但未显示出明显变化。通过使用HPLC对鲣鱼油中的25-羟基-D3进行了测定，测定值为1.8微克/克。这比维生素D3的含量低约1/800。
