Erickson R P, Ferrucci S, Rahe B, Rosenberg M P, Morello D
Mol Biol Rep. 1984 Dec;10(2):109-13. doi: 10.1007/BF00776983.
We have investigated differences in C pG methylation between F9 embryonal carcinoma cells in vitro and as tumor cells grown in vivo using Msp I and Hpa II restriction isoschizomers. Southerns were hybridized with two low copy number probes, mouse major beta-globin (f7) and a class I, histocompatibility-2 cDNA clone (pH-2d-4). In each case, the tumor-DNA was hypomethylated while the DNA from F9 cells grown in vitro was moderately methylated. We conclude that growth conditions or cell-cell interactions can greatly affect methylation of C pG sites.
我们使用Msp I和Hpa II同裂酶研究了体外培养的F9胚胎癌细胞与体内生长的肿瘤细胞之间CpG甲基化的差异。用两个低拷贝数探针，即小鼠主要β-珠蛋白（f7）和一个I类组织相容性-2 cDNA克隆（pH-2d-4）对Southern印迹进行杂交。在每种情况下，肿瘤DNA均发生低甲基化，而体外培养的F9细胞的DNA则发生中度甲基化。我们得出结论，生长条件或细胞间相互作用可极大地影响CpG位点的甲基化。
