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一种编码小T抗原但不编码中T抗原的多瘤病毒突变体表现出与细胞转化相关的细胞表面和细胞骨架变化的解偶联。

A polyoma mutant that encodes small T antigen but not middle T antigen demonstrates uncoupling of cell surface and cytoskeletal changes associated with cell transformation.

作者信息

Liang T J, Carmichael G G, Benjamin T L

出版信息

Mol Cell Biol. 1984 Dec;4(12):2774-83. doi: 10.1128/mcb.4.12.2774-2783.1984.

DOI:10.1128/mcb.4.12.2774-2783.1984
PMID:6098822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369288/
Abstract

The hr-t gene of polyoma virus encodes both the small and middle T (tumor) antigens and exerts pleiotropic effects on cells. By mutating the 3' splice site for middle T mRNA, we have constructed a virus mutant, Py808A, which fails to express middle T but encodes normal small and large T proteins. The mutant failed to induce morphological transformation or growth in soft agar, but did stimulate postconfluent growth of normal cells. Cells infected by Py808A became fully agglutinable by lectins while retaining normal actin cable architecture and normal levels of extracellular fibronectin. These properties of Py808A demonstrated the separability of structural changes at the cell surface from those in the cytoskeleton and extracellular matrix, parameters which have heretofore been linked in the action of the hr-t and other viral oncogenes.

摘要

多瘤病毒的hr-t基因编码小T和中T（肿瘤）抗原，并对细胞产生多效性影响。通过突变中T mRNA的3'剪接位点，我们构建了一种病毒突变体Py808A，它不能表达中T，但能编码正常的小T和大T蛋白。该突变体不能诱导形态转化或在软琼脂中生长，但能刺激正常细胞汇合后的生长。被Py808A感染的细胞在用凝集素处理时变得完全可凝集，同时保持正常的肌动蛋白丝结构和细胞外纤连蛋白的正常水平。Py808A的这些特性表明细胞表面结构变化与细胞骨架和细胞外基质中的结构变化是可分离的，而迄今为止，在hr-t和其他病毒癌基因的作用中，这些参数是相互关联的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/8db336d861a8/molcellb00154-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/db5e6b5f1255/molcellb00154-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/737c071bcf33/molcellb00154-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/95536443ab47/molcellb00154-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/8db336d861a8/molcellb00154-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/db5e6b5f1255/molcellb00154-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/737c071bcf33/molcellb00154-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/95536443ab47/molcellb00154-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b19b/369288/8db336d861a8/molcellb00154-0223-a.jpg

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1
A polyoma mutant that encodes small T antigen but not middle T antigen demonstrates uncoupling of cell surface and cytoskeletal changes associated with cell transformation.一种编码小T抗原但不编码中T抗原的多瘤病毒突变体表现出与细胞转化相关的细胞表面和细胞骨架变化的解偶联。
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A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.β0地中海贫血基因剪接位点的单碱基变化导致异常的RNA剪接。
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