Montell C, Fisher E F, Caruthers M H, Berk A J
Nature. 1982 Feb 4;295(5848):380-4. doi: 10.1038/295380a0.
Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as two overlapping spliced mRNAs, 12S and 13S, which encode closely related proteins. To distinguish between the functions of these proteins, a single T leads to G transversion was constructed which prevents splicing of the 12S mRNA. This transversion, in the second base of the 12S mRNA intron, does not alter the protein encoded by the 13S mRNA due to degeneracy in the genetic code. Studies with this mutant demonstrated that only the 13S mRNA encodes the regulatory protein required for normal early gene expression.
人类腺病毒的早期区域IA编码正常诱导早期病毒基因和病毒诱导细胞转化所需的一种功能。该区域在早期以两种重叠的剪接mRNA，即12S和13S的形式表达，它们编码密切相关的蛋白质。为了区分这些蛋白质的功能，构建了一个单一的T到G颠换，该颠换阻止了12S mRNA的剪接。这种位于12S mRNA内含子第二个碱基处的颠换，由于遗传密码的简并性，不会改变由13S mRNA编码的蛋白质。对该突变体的研究表明，只有13S mRNA编码正常早期基因表达所需的调节蛋白。
