Browne G M, Skotnicki M L, Goodman A E, Rogers P L
Plasmid. 1984 Nov;12(3):211-4. doi: 10.1016/0147-619x(84)90047-7.
The transformation of Zymomonas mobilis by plasmid DNA was achieved using a modification of the CaCl2 method for Escherichia coli. The highest frequency of transformation obtained was 5 X 10(3) transformants/micrograms DNA. The success of the method depended upon the use of a plasmid which is a cointegrate between a Z. mobilis cryptic plasmid and an E. coli plasmid carrying two selectable drug resistance markers.
