运动发酵单胞菌中携带抗生素抗性的自我传递质粒。
Self-transmissible plasmid in Zymomonas mobilis carrying antibiotic resistance.
作者信息
Walia S K, Carey V C, All B P, Ingram L O
出版信息
Appl Environ Microbiol. 1984 Jan;47(1):198-200. doi: 10.1128/aem.47.1.198-200.1984.
The cryptic plasmid pRUT41 from Zymomonas mobilis was examined for its biological properties. This plasmid was found to be conjugally transferred from Z. mobilis CP4 to Escherichia coli BM21 and to carry genes for antibiotic resistance (gentamicin, kanamycin, and streptomycin). Covalently closed circular plasmid DNA was isolated from eight transconjugants of E. coli BM21. These plasmids were identical in mobility on agarose gels and exhibited the same restriction patterns as the native pRUT41 plasmid isolated from Z. mobilis. The plasmid location of the antibiotic resistance genes was further confirmed by transforming E. coli BM21 with isolated pRUT41 plasmid from strain CP4 and with plasmids from the transconjugants of BM21. Resistance to streptomycin, kanamycin, and gentamicin was tightly linked and transferred together in all cases.
对来自运动发酵单胞菌的隐蔽质粒pRUT41的生物学特性进行了研究。发现该质粒可从运动发酵单胞菌CP4接合转移至大肠杆菌BM21，并携带抗生素抗性基因（庆大霉素、卡那霉素和链霉素）。从大肠杆菌BM21的八个接合子中分离出共价闭合环状质粒DNA。这些质粒在琼脂糖凝胶上的迁移率相同，并且与从运动发酵单胞菌中分离出的天然pRUT41质粒具有相同的限制性酶切图谱。通过用从CP4菌株分离的pRUT41质粒以及从BM21接合子中获得的质粒转化大肠杆菌BM21，进一步证实了抗生素抗性基因的质粒定位。在所有情况下，对链霉素、卡那霉素和庆大霉素的抗性紧密连锁并一起转移。
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