Fang R X, Cai F X, Tian Y C, Bu M, Xi Z X, Mang K Q
Sci Sin B. 1984 Oct;27(10):1015-22.
The genomic DNA of CaMV (Xinjiang isolate) was mapped on the virion DNA with a number of restriction endonucleases by double digestions and partial digestions of one-end 32P-labelled fragments. It contained the unique sites of BglI, SalI and XhoI. BamHI and HhaI each cut it at two sites, EcoRI at five sites (three of which were located), HpaII at seven sites, BglII at eight sites, and HaeIII at ten sites. The sites of all the enzymes used above, as well as HindIII which cut at ten sites were also mapped on the cloned CaMV DNA. Virion DNA and cloned viral DNA gave the same results in the number and location of cleavage sites of the restriction enzymes tested on both DNAs. Like most of the other isolates, CaMV-Xinjiang isolate has three single-stranded discontinuities. From the digestion patterns of SalI, XhoI, EcoRI and HhaI, it is concluded that the restriction map of CaMV-XJ genome is closely related to that of BKMV isolate from East Germany.
通过对一端用32P标记的片段进行双酶切和部分酶切，用多种限制性内切酶将花椰菜花叶病毒（新疆分离株）的基因组DNA定位到病毒粒子DNA上。它含有BglI、SalI和XhoI的独特位点。BamHI和HhaI各在两个位点切割它，EcoRI在五个位点切割（其中三个位点已定位），HpaII在七个位点切割，BglII在八个位点切割，HaeIII在十个位点切割。上述所有使用的酶的位点，以及在十个位点切割的HindIII的位点也定位在克隆的花椰菜花叶病毒DNA上。病毒粒子DNA和克隆的病毒DNA在两种DNA上测试的限制性酶切位点的数量和位置上给出了相同的结果。像大多数其他分离株一样，花椰菜花叶病毒新疆分离株有三个单链间断。从SalI、XhoI、EcoRI和HhaI的酶切图谱可以得出结论，花椰菜花叶病毒新疆分离株基因组的限制性图谱与来自东德的BKMV分离株的限制性图谱密切相关。
