Naroditskiĭ B S, Roninson I B, Kislina O S, Zavizion B A, Tikhonenko T I
Mol Biol (Mosk). 1979 Mar-Apr;13(2):309-23.
The effect of 7 specific endonucleases EcoRI, BamHI, BglII, SalI HindIII, XhoI, SmaI, on the genome of the phage Sd was studied. The molecular weight of the genome was estimated as 45.10(6). BamHI, EcoRI, HindIII, BglII, SmaI have altogether 15 sites of restriction SalI and XhoI do not hydrolyse the phage DNA. Fragmentation of the phage DNA in conditions of partial hydrolysis and simultaneous action of several enzymes have allowed to draw a physical man to the phage Sd DNA is more resistent to the action of restriction enzymes than DNAs from other phages. The mean size of Sd DNA fragments exceeds the statistical value almost by one order of magnitude.
研究了7种特异性核酸内切酶EcoRI、BamHI、BglII、SalI、HindIII、XhoI、SmaI对噬菌体Sd基因组的作用。基因组的分子量估计为45.10(6)。BamHI、EcoRI、HindIII、BglII、SmaI共有15个限制性位点，SalI和XhoI不水解噬菌体DNA。在部分水解和几种酶同时作用的条件下，噬菌体DNA的片段化使得能够绘制出噬菌体Sd的物理图谱。与来自其他噬菌体的DNA相比，噬菌体Sd的DNA对限制性酶的作用更具抗性。Sd DNA片段的平均大小几乎超过统计值一个数量级。
