Marzoev A I, Maksina A G, Borshchevskaia T A
Vopr Med Khim. 1984;30(6):21-4.
Structural and functional characteristics of sarcoplasmic reticulum vesicles (SRV) from skeletal muscle of normal and hypothyroid rabbits were investigated. The rate of Ca2+ absorption was decreased in hypothyroid animals and Ca2-ATPase activity in SRV of these animals was lower than that of control rabbits. The transport ratio (Ca2+/ATP) in hypothyroid rabbits was higher by 20% than in controls. In SRV of hypothyroid animals a decrease in the protein/lipid interaction and higher fluidity of membrane lipids were observed. Administration of L-thyroxine (300 mg/kg of body mass, intraperitoneally) normalized the SR lipids fluidity within about 40 hrs after the injection but did not affect significantly the patterns of protein/lipid interaction. Normal and hypothyroid SRV were similar in their protein spectrum as shown by electrophoresis in polyacrylamide gel. These alterations observed in the SRV structure and function are apparently related to modifications of membrane lipids.
对正常和甲状腺功能减退兔子骨骼肌肌浆网囊泡（SRV）的结构和功能特性进行了研究。甲状腺功能减退动物的Ca2+吸收速率降低，这些动物SRV中的Ca2-ATP酶活性低于对照兔子。甲状腺功能减退兔子的转运比（Ca2+/ATP）比对照组高20%。在甲状腺功能减退动物的SRV中，观察到蛋白质/脂质相互作用减少，膜脂流动性增加。腹腔注射L-甲状腺素（300mg/kg体重）后约40小时，SR脂质流动性恢复正常，但对蛋白质/脂质相互作用模式无显著影响。如聚丙烯酰胺凝胶电泳所示，正常和甲状腺功能减退的SRV在蛋白质谱方面相似。在SRV结构和功能中观察到的这些变化显然与膜脂的修饰有关。
