Senault C, Le Comte V, Portet R
Biochimie. 1984 Jul-Aug;66(7-8):573-8. doi: 10.1016/0300-9084(84)90153-6.
In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristics in both types of preparation; competition studies of [3H] dihydroalprenolol binding led to the characterization of beta 1 subtype adrenergic receptors with a lower affinity of beta-adrenergic agonists for [3H] dihydroalprenolol binding sites in membranes than that found in isolated cells. Cold acclimation produced, in isolated cells only, a decrease of 41% in the [3H] dihydroalprenolol binding sites and a beta-adrenergic agonist affinity increase. It is concluded that beta-adrenergic receptor decrease could be a factor, at the hormone receptor interaction level, in the regulation of the transmission of biological action responsible for the cold-induced decrease in catecholamine responsiveness in brown adipose tissue. For a study of the desensitization process in brown fat, isolated cells seem to offer certain advantages over a crude membrane preparation.
关于在冷适应大鼠棕色脂肪体外观察到的对儿茶酚胺代谢敏感性降低的情况，在大鼠肩胛间棕色脂肪组织的分离细胞和粗制膜制剂中研究了β - 肾上腺素能受体。[3H]二氢阿普洛尔结合在两种制剂类型中具有相同的特征；[3H]二氢阿普洛尔结合的竞争研究导致β1亚型肾上腺素能受体的表征，与在分离细胞中发现的相比，β - 肾上腺素能激动剂对膜中[3H]二氢阿普洛尔结合位点的亲和力较低。冷适应仅在分离细胞中使[3H]二氢阿普洛尔结合位点减少41%，并使β - 肾上腺素能激动剂亲和力增加。结论是，β - 肾上腺素能受体减少可能是在激素受体相互作用水平上，调节负责棕色脂肪组织中冷诱导儿茶酚胺反应性降低的生物作用传递的一个因素。对于棕色脂肪脱敏过程的研究，分离细胞似乎比粗制膜制剂具有某些优势。
