Gouardères C, Cros J
Neuropeptides. 1984 Dec;5(1-3):113-6. doi: 10.1016/0143-4179(84)90040-4.
Opioid receptor binding sites were analyzed in various segments of rat spinal cord. Mu and delta types were labelled with [3H]-DHM or [3H]-DAGO and [3H]-DADLE or [3H]-DSLET respectively. Kappa 1 (kappa) and kappa 2 (benzomorphan) binding sites were individually detected by the overall labeling of opioid binding sites with [3H]-etorphine followed by the elimination of binding to particular sites by the use of appropriate selective unlabelled ligands. Whereas lumbo sacral region contained mainly the kappa 2 site, thoracic membranes had a proportion of approximately 20% mu, 20% kappa 1 and 60% kappa 2 and cervical region contained much less kappa 2 sites (25% mu, 20% delta, 28% kappa 1 and 32% kappa 2).
对大鼠脊髓的不同节段中的阿片受体结合位点进行了分析。μ型和δ型分别用[³H]-DHM或[³H]-DAGO以及[³H]-DADLE或[³H]-DSLET进行标记。κ1（κ）和κ2（苯并吗啡烷）结合位点通过用[³H]-埃托啡对阿片结合位点进行整体标记，然后使用适当的选择性未标记配体消除与特定位点的结合来分别检测。腰荐部区域主要含有κ2位点，胸段膜含有约20%的μ型、20%的κ1型和60%的κ2型，而颈部区域含有的κ2位点要少得多（25%的μ型、20%的δ型、28%的κ1型和32%的κ2型）。
