Klein R J, Kaley L A, Friedman-Kien A E
Vaccine. 1984 Sep;2(3):219-23. doi: 10.1016/0264-410x(84)90089-6.
Immunization of hairless mice with a TK-, ACVr, non-pathogenic herpes simplex virus (HSV) type 1 mutant protected the mice against reinfection with lethal doses of the parental pathogenic HSV strain. The protection conferred by the mutant against the establishment of latency after reinfection with the parental strain was dependent on the site of reinfection; after reinfection at the same site, only 2% of mice became latently infected, compared to 32% after reinfection at a distant site. When inoculation with the mutant was done at two different sites, single reinfections at any site led to the establishment of latency in 4% of the mice. The mutant by itself was almost completely latency-negative: only 4.5% of the mice developed latency in trigeminal ganglia and 2.3% in the spinal ganglia. The rate of mutant-induced latent infections is partly related to the dose of the virus; however, lower doses of the mutant may not colonize the ganglia, and therefore fail to protect against challenge infections.
用一种缺失胸苷激酶（TK）、对阿昔洛韦耐药（ACVr）的非致病性单纯疱疹病毒1型（HSV）突变株对无毛小鼠进行免疫接种，可保护小鼠免受致死剂量的亲本致病性HSV毒株的再次感染。该突变株在亲本毒株再次感染后对潜伏感染建立的保护作用取决于再次感染的部位；在同一部位再次感染后，只有2%的小鼠发生潜伏感染，而在远处部位再次感染后这一比例为32%。当在两个不同部位接种突变株时，在任何部位进行单次再次感染会导致4%的小鼠发生潜伏感染。该突变株本身几乎完全不形成潜伏感染：只有4.5%的小鼠在三叉神经节形成潜伏感染，2.3%的小鼠在脊髓神经节形成潜伏感染。突变株诱导潜伏感染的发生率部分与病毒剂量有关；然而，较低剂量的突变株可能无法在神经节中定植，因此无法预防攻击感染。
