Klein R J, Buimovici-Klein E, Moser H, Moucha R, Hilfenhaus J
Arch Virol. 1981;68(2):73-80. doi: 10.1007/BF01314437.
Hairless mice were immunized with herpes simplex virus type 1 (HSV-1) envelope antigen (EAG), EAG in association with polyriboinosinic . polyribocytidylic acid-poly-L-lysine complexed with carboxymethylcellulose (PICLC), and inactivated purified HSV-1 (VAG). After 2 weeks the mice were challenged by a percutaneous HSV-1 infection in the orofacial (OF) or lumbosacral (LS) skin area. Following immunization a consistent cell-mediated immune response was observed in all immunized mice, although the humoral immune response was very low, or not detectable. After challenge, a marked secondary humoral and cell-mediated immune response developed in all immunized mice, and the animals were protected against the development of skin lesions and the fatal outcome of infection. However, the establishment of latent infections in the sensory ganglia was not prevented by the immunization procedure.
用1型单纯疱疹病毒（HSV-1）包膜抗原（EAG）、与聚肌苷酸.聚胞苷酸-聚-L-赖氨酸复合的羧甲基纤维素（PICLC）结合的EAG以及灭活的纯化HSV-1（VAG）对无毛小鼠进行免疫。2周后，通过经皮HSV-1感染在口面部（OF）或腰骶部（LS）皮肤区域对小鼠进行攻击。免疫后，在所有免疫小鼠中均观察到一致的细胞介导免疫反应，尽管体液免疫反应非常低或无法检测到。攻击后，所有免疫小鼠均出现明显的二次体液和细胞介导免疫反应，并且动物受到保护，防止皮肤病变的发展和感染的致命结局。然而，免疫程序并未阻止感觉神经节中潜伏感染的建立。
