Westaway E G, Speight G, Endo L
Virus Res. 1984;1(4):333-50. doi: 10.1016/0168-1702(84)90022-4.
The rates of inactivation of synthesis of individual virus-specified proteins by ultraviolet radiation provided an estimate of the target sizes of individual viral genes. In a control experiment with Semliki Forest virus, the genes for the structural proteins mapped in the known sequence 5' C-PE2-E1 3', and in accordance with initiation of translation from a single site on 26-S mRNA. Under the same conditions, the inactivation of synthesis of seven Kunjin virus-specified proteins also followed first-order kinetics, but the largest target size was equivalent to only about half the length of the genome. No gene sequence could be deduced using the premise that translation was initiated at a single site. However, genes for the major nonstructural proteins could be mapped in the order 5' P98-P71-P10 3' from a postulated ribosomal attachment site about midway along the RNA. The genes for the structural proteins C and E could then be mapped in a preceding sequence 5'...C-E-GP19-P21 3', with a vacant upstream region sufficient to code for at least one of two flavivirus genes not expressed in these experiments. The implications of the two postulated translational units are discussed in relation to previous data on translation strategy of flaviviruses.
紫外线辐射对单个病毒特异性蛋白质合成的失活速率提供了单个病毒基因靶标大小的估计。在用辛德毕斯病毒进行的对照实验中，结构蛋白的基因按已知序列5'-C-PE2-E1-3'定位，并与从26-S mRNA上的单个位点开始翻译一致。在相同条件下，七种库京病毒特异性蛋白质合成的失活也遵循一级动力学，但最大的靶标大小仅相当于基因组长度的一半左右。基于翻译从单个位点开始这一前提无法推导出基因序列。然而，主要非结构蛋白的基因可以从沿着RNA大约中间位置的一个假定核糖体附着位点按5'-P98-P71-P10-3'的顺序定位。然后结构蛋白C和E的基因可以定位在前面的序列5'-...C-E-GP19-P21-3'中，上游有一个空缺区域，足以编码在这些实验中未表达的两种黄病毒基因中的至少一种。结合先前关于黄病毒翻译策略的数据讨论了这两个假定翻译单元的意义。
