Coia G, Parker M D, Speight G, Byrne M E, Westaway E G
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
J Gen Virol. 1988 Jan;69 ( Pt 1):1-21. doi: 10.1099/0022-1317-69-1-1.
A Kunjin (KUN) virus cDNA sequence of 10664 nucleotides was obtained and it encoded a single open reading frame for 3433 amino acids. Partial N-terminal amino acid analyses of KUN virus-specified proteins identified the polyprotein cleavage sites and the definitive gene order. The gene order relative to that proposed for yellow fever (YF) virus is as follows: KUN 5'-C.GP20.E.GP44.P19.P10.P71.(?).P21.P98-3' YF 5'-C.prM.E.NS1.ns2a.ns2b.NS3.ns4a.ns4b. NS5-3'. The order of putative signal sequences and stop transfer sequences indicated that KUN NS1, NS2A and NS4B are probably cleaved in the lumen of the endoplasmic reticulum, at a consensus site Val-X-Ala decreases where X is an uncharged residue, and NS2B, NS3 and NS5 are cleaved in the cytosol at the site Lys-Arg decreases Gly. Comparisons with the complete amino acid sequences of YF and West Nile (WN) viruses showed that KUN virus shared 93% homology with WN virus, but only 46% homology with YF virus. Comparisons among individual gene products of six flaviviruses showed that E, NS1, NS3 and NS5 tended to be the most highly conserved, and C among the least conserved. Homologous cleavage sites were evident, and six domains in NS5, a total of over 170 residues, shared at least 85% homology. Comparisons with the KUN C to NS2B sequence defined a gradient of relationships of all gene products in decreasing order WN greater than Murray Valley greater than Japanese encephalitis greater than St Louis encephalitis viruses within this closely related serological complex. A non-coding 5' sequence (75 nucleotides) of KUN virus shared 95% homology with WN virus and a shorter imperfect match with Murray Valley encephalitis virus (15 of 18 nucleotides). The KUN non-coding 3' sequence of 290 nucleotides contained several short and imperfectly matched sequences, and shared 87% homology over the distal region of 191 nucleotides with the corresponding region of WN virus RNA.
获得了一条10664个核苷酸的库宁（KUN）病毒cDNA序列，它编码一个由3433个氨基酸组成的单一开放阅读框。对KUN病毒特异性蛋白的部分N端氨基酸分析确定了多蛋白切割位点和明确的基因顺序。相对于黄热（YF）病毒提出的基因顺序如下：KUN 5'-C.GP20.E.GP44.P19.P10.P71.(?).P21.P98-3' YF 5'-C.prM.E.NS1.ns2a.ns2b.NS3.ns4a.ns4b.NS5-3'。推测的信号序列和终止转移序列的顺序表明，KUN NS1、NS2A和NS4B可能在内质网腔内的一个共有位点Val-X-Ala（其中X是一个不带电荷的残基）处被切割，而NS2B、NS3和NS5在细胞质中在Lys-Arg处被切割（减少Gly）。与YF和西尼罗河（WN）病毒的完整氨基酸序列比较表明，KUN病毒与WN病毒有93%的同源性，但与YF病毒只有46%的同源性。六种黄病毒的各个基因产物之间的比较表明，E、NS1、NS3和NS5往往是最保守的，而C是最不保守的。同源切割位点很明显，NS5中的六个结构域，总共超过170个残基，共享至少85%的同源性。与KUN从C到NS2B的序列比较确定了在这个密切相关的血清学复合体中，所有基因产物的关系梯度，顺序为WN大于墨累谷大于日本脑炎大于圣路易斯脑炎病毒。KUN病毒的一个非编码5'序列（75个核苷酸）与WN病毒有95%的同源性，与墨累谷脑炎病毒有较短的不完全匹配（18个核苷酸中的15个）。KUN病毒290个核苷酸的非编码3'序列包含几个短的且不完全匹配的序列，并且在191个核苷酸的远端区域与WN病毒RNA的相应区域有87%的同源性。
